Hello and welcome to my blog! My name is Caroline and I am a PhD student at the University of Sheffield. My research project focuses on Striga - a genus of parasitic plants that devastates harvests by infecting food crops. I am exploring the defence reactions that can make host plants more resistant against Striga. Due to my ongoing battles with anorexia, I haven't made as much progress as I would have liked but I am determined to finish the course.
This blog charts the ups and downs of life in the lab, plus my dreams to become a science communicator and forays into public engagement and science policy....all while trying to keep my mental and physical health intact. Along the way, I'll also be sharing new plant science stories, and profiles of some of the researchers who inspire me on this journey. So whether you have a fascination for plants, are curious about what science research involves, or just wonder what exactly I do all day, read on - I hope you find it entertaining!
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Thursday, 6 November 2014
Getting it off my Chest...
So what did I decide it talk about? GM plants. I believe that it is irresponsible for the government to ignore the potential of this technology to address key concerns including food security, climate change and the energy crisis. I also feel that public opinion has been allowed to turn against GM technology on the basis of vigorous anti- campaign groups rather than a balanced representation of the facts. I do not mind if someone is against GM crops, as long as they have had all the information to make an informed judgement. And I agree that the technology needs to be demonstrated to be safe before applied on a commercial agricultural scale. So what do I want?
* for the government to invest in transferring Gm technology from the lab to the field, including extensive field trials to evaluate safety risks, etc.
* for greater representation of he potential benefits of GM technology in public media outlets and also a platform for wider debate
No doubt, not everyone reading this will agree. For myself, I am surprised that the Green Party is so decided against GM crops when one of their manifestos is to reduce the application of pesticides on crops. Using GM technology to engineer plants with highly tailored, innate resistance against specific pests only, could avoid the blanket use of pesticides which devastate so much natural wildlife. Surely GM crops can't be an entirely bad thing if they help to preserve the native bees, bugs and butterflies that our biodiversity depends on?
Rant over! If you want to join in, comments below please!
Sunday, 2 November 2014
The gentle art of tweaking...and an update on Green Tea
Unfortunately, research (at least with living organisms) is rarely a case of simply drafting a hypothesis, planning an experiment to investigate it and then just DOING it. So much time (especially for PhD students) is spent fiddling with different conditions, trying to coddle their model organism of choice into growing or doing what is required. In my case, I need my seedlings to be robust enough to survive being transplanted into the rhizotrons where I can infect them with the Striga parasite. My first suspect is the wind speed in the growing chamber - I can see the poor seedlings trembling with each blast of air and Arabidopsis is known for not liking wind. Unfortunately, it is very difficult to adjust the settings on the Conviron cabinet so my supervisor has said she will help me construct a windbreak! If that doesn't help, I will try altering the compost and also the vernalisation period - Arabidopsis seeds only germinate if they have been exposed to the cold (which allows seeds to remain dormant over winter) so we "wake them up" by putting them in the fridge for 48 hours. Perhaps this isn't long enough?
Meanwhile, following on from my previous post ("Ask for Evidence"), I have been investigating the merits of Green Tea. It turns out that there IS quite a bit of scientific evidence that drinking green tea could help prevent Alzheimer's. The disease is caused by accumulations of beta-amyloid protein which form plaques that cause neurodegeneration. Compounds in green tea called catechins, however, are thought to inhibit this process. Specifically, epigallocatechin-3-gallate (EGCG) prevents copper and zinc ions from binding to amyloid proteins, which prevents plaque formation. In a transgenic mouse model engineered to display early-onset Alzheimer's, animals that received EGCG performed better in learning/memory tasks (such as finding a hidden platform in a water tank) and had fewer deposits of amyloid plaques in the brain. Even more amazingly, a further study found that, besides countering neuronal decay, EGCG can actually promote the generation of new neurones in the hippocampus, the area of the brain associated with converting short-term memories to long-term ones).
So if you want to raise a glass to your health, make it Green Tea! Cheers!
For more information see:
http://www.lef.org/magazine/2014/8/how-green-tea-protects-against-alzheimers-disease/page-01
Epigallocatechin-3-Gallate ameliorates learning and memory deficits by adjusting the balance of TrkA/p75NTR signaling in APP/PS1 transgenic mice. Molecular Neurobiology, December 2013
Green tea epigallocatechin-3-gallate (EGCG) promotes neural progenitor cell proliferation and sonic hedgehog pathway activation during adult hippocampal neurogenesis. Molecular Nutrition & Food Research, August 2012
Tuesday, 21 October 2014
Consumers unite! And - Ask for Evidence!
How many times has your grocery shopping been influenced by a product's health claim? Do you trust that Government policies are driven by rigorous, scientific evidence? Do you ever wonder where economists get their forecasts from?
And if you are unsure, how can you find out?
Yesterday, I was fortunate enough to attend a highly entertaining seminar held by the University of Sheffield's very own Science in Policy group. The speaker was Dr Chris Peters, Scientific Liaison at 'Sense about Science'.
Every day we face a barrage of claims designed to make us spend money, change our behaviour or support a particular policy. But how can we tell if these are true? What is to stop me from inventing my own smoothie concoction, perhaps using some of my surplus Arabidopsis plants, and claiming that it increases concentration by 20% and improves life expectancy by 3.5 years?
In short, not a lot. It isn't illegal to make such claims and the Advertising Standards Agency, whilst powerful, is only a small organisation that can barely keep up with the unending flow of wonderful promises.
Ten years ago, a group of scientists led by Lord Taverne and Tracey Brown decided this wasn't quite right and set up 'Sense about Science'. This has since grown to a current network of over 5,000 scientists encompassing the entire breadth of expertise, from Nobel prize winners to PhD students and early career scientists. The main aim, in particular for the 'Ask for Evidence' campaign, is to hold companies to account for the promises they make about their products and policies. It involves, quite simply, asking where the evidence behind their claim comes from. The beauty of this camping is its simplicity, which enables anyone, scientist or not, to take part.
Once you start asking where the evidence is, you'll find yourself questioning all sorts of things. In one case, the campaign questioned a bold claim by the Daily Telegraph that wind turbines could 'knock tens of thousands of pounds off the value of nearby homes' and that a nearby turbine could 'knock eight per cent off average home value'. After a bit of digging, it turned out that this very precise figure was merely based on a single estate agent's opinion. During his talk, Chris also outlined the case of a mother of two children who challenged her local leisure centre's policy that children under the age of eight had to be supervised in a 1:1 ratio. This strict rule, taken up by a number of facilities, was brought into being simply because the management 'just thought' it was a safe approach! Other examples have seen products pulled off the shelves and have debunked claims about health- enhancing properties. It is largely thanks to their work that newspaper articles do now usually provide a reference for their story. Further successes can be found on the website: http://www.senseaboutscience.org/pages/a4e_examples_of_evidence_hunting.html
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| Me and Dr Chris Peters with the famous sign - last held by Jonathan Ross apparently! |
But Chris is keen for more people to be involved. So if you spot an unqualified claim, why not challenge it? Send the company a letter, give their head office a ring or download the online 'Ask for Evidence' tool which allows you to upload photographs of the ( potentially bogus ) claim. Just think what you can expose and how satisfying it would be to protect the public from misinformation. On a serious note, the 'All Trials' campaign also seeks to improve the registering and reporting of clinical trials so that drugs companies cannot simply select the most favourable trial results when promoting their product. This is vital to ensure that patients don't pin their hopes and spend a fortune on a flawed treatment. As an example, the UK Government spent £424 million pounds stockpiling the Tamiflu drug as a precaution against Swineflu, even though it is now thought that paracetamol is just as effective. In such cases, a lack of evidence can cost a fortune, and even lives.
So next time you suspect that someone is trying to pull the wool over your eyes, remember that it is in your power to act! Check out the website at http://www.senseaboutscience.org/pages/a4e.html
As for me, I have been led along by the claim that green tea can help prevent Alzheimer's. But DOES IT? I think it is time to...ask for evidence! Watch this space!
Thursday, 16 October 2014
A bit of PLANT DIY...
| Welcome to my chamber ...Conviron 502, waiting to be filled with plants |
Wednesday, 24 September 2014
A different 'plant scientist' - Ron Finley, 'Gangsta Gardener'
A former fashion designer, Finley became exasperated at the impoverished state of the communities around his home in South Central, USA. Not that there was a lack of food - instead, streets of houses were shoehorned by fast- food outlets, supermarkets and diners. Nevertheless, there was a distinct lack of nutritious foodstuffs - fresh fruit and vegetables. To Finley's mind, this food imbalance was the key underlying cause of the rise in chronic 'Western' diseases such as obesity, diabetes and heart disease. He saw that he and his neighbours lived in a 'food prison' , allowing themselves to depend entirely on others for food. But how to escape?
Besides fast food eateries, South Central also has a lot of vacant lots, areas of wasteland neglected by local authorities. On one such strip outside his house, Finley daringly planted tomatoes, carrots, mustard greens, sunflowers and more, turning the street into a garden. Despite the beauty and bounty this provided, a complaint from a neighbour led to Finley being arrested. This prompted a successful campaign which changed the local law, allowing citizens to grow produce on their parkways.
Finley was evangelical in his message that this was more than about growing a few vegetables to enhance his own meals. Instead, he deliberately planned the street garden so that neighbours would be able to help themselves as they passed. His vision is for neighbourhoods to feed themselves, growing and sharing vegetables on community land. To him, this is the ultimate act of defiance - fighting back against the reliance on mass- produced, processed food.
Since then, Finley and his 'gangsta gardeners' have worked with various communities to set up new street gardens. It is clear that these have had effects beyond improving nutrition, especially in helping children to engage with nature. 'Gardens can teach so many lessons' Finley said 'everything in life happens in a garden'. Time and again, he has observed young people become 'seduced by the soil' and his gardens have proved therapeutic for those suffering from substance abuse, mental and physical conditions and the pressures of gang warfare. Once children start growing healthier foods, they also become much more enthusiastic about incorporating them into their diets.
Finley distinguishes himself from guerrilla gardeners ( those that plant seeds / gardens at midnight then vanish before morning), instead styling himself as a 'gangsta gardener'. The key difference, he says, is stewardship. He only works with communities that are prepared to take ownership of their garden and maintain it. During the question period that followed, a representative of a Sheffield Council also made the point that it was no good for the council to 'do things at people' but that sustainable community initiatives had to be undertaken by the people themselves. The overriding message appeared to be that councils are more supportive than the public may think and are prepared to back ideas that people are committed to maintain themselves. In fact, a new 'urban garden' is being planned for Sheffield City centre, called 'the Love Square'. Fingers crossed it will have the same effects as Finley's Garden! ( see http://lovesquare.group.shef.ac.uk )
According to Finley, ' they are telling us a lie, that we need them to feed us'. As a plant scientist, I found this a little hard to stomach, having been brought up to believe that sustainable, mass agricultural systems are the most efficient way to feed the global population. However, I believe that the world would be a healthier and happier place if most people did grow their own food. Perhaps a community in the UK could sustain itself on produce grown and reared by its members? Nevertheless, there is also a compelling argument that the 'delegation of labour' frees other people to become inventors, medical researchers, technicians, artists, poets... Perhaps a mix of both approaches is best?
So what do people think? Is mass produced agriculture a glory of the modern world or leading many to an early grave? Comments below please!
Click hear for a Reader's Digest interview with Ron Finley and a video of his 'Gospel Message' http://www.rd.com/culture/ron-finley-gangsta-gardener/#.
Thursday, 18 September 2014
Genes for Jeans day tomorrow!
Jeans for Genes originated in the 1990s, as part of a family's efforts to raise funds for The Chronic Granulomatous Disorder Society. Two brothers within the family were affected by this condition which hinders the ability of the body's immune cells to kill pathogens. The idea has since grown into a national event to raise money for Genetic Disorders UK, which supports families affected by genetic conditions. This year, Jeans for Genes has worked with Edinburgh College of Art to produce a series of limited edition T-Shirts. To me, the design is a perfect combination of promoting my scientific interests whilst supporting a valuable cause. It is also brilliant to use science to make a fashion statement! Or should that be the other way around?
With so many pressures facing our world - food security, health, energy generation- scientists often have to stretch their creative powers to garner enough funds to persue their work. It is difficult when there are so many promising avenues of research for funding bodies to decide between them.
But the public can play their part so I shall wear my T Shirt with pride.
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| Jeans for Genes Day T-Shirt Design 2014 |
If it's too late this year for you to organise an event with your colleagues, why not resolve now to wear your genes on Jeans for Genes Day 2015?
For more information see http://www.jeansforgenesday.org
Friday, 5 September 2014
Another year, another name badge! The Annual Gatsby Plants Network Meeting 4-5 th September 2014
This year the venue is Somerville College, Oxford. Although the grounds are lovely and bursting with an abundance of floral species, I am slightly disappointed that we are housed in a modern university block...I had got used to the quirky college rooms with their nooks, crannies and occasionally pianos. Nevertheless, it doesn't detract for the science, which- as usual- is top notch. Thursday afternoon is mostly dedicated to talks from Gatsby funded PhD students encompassing everything from micro compartmentalisation of the enzyme aldolase, chromosome recombination in Arabidopsis and resistance against the pathogenic oomycete Albugo laibachi. I always struggle to get my head around some of the development talks, particularly how polarity fields and hormone gradients can promote the growth of specific shapes. Some ingenious comparative models were used in the talks to explain such concepts, including pizza dough and lolly pops. Visual imagery always helps! I was also intrigued by an experimental method called FRET to determine if two proteins interact in a cell. By altering the encoding gene, it is possible to engineer proteins to have a fluorescent 'tag' which absorbs a certain light wavelength and re- emits some of the energy as fluorescent light. Some of the energy, however, can be transferred to surrounding molecules (known as Fluorescence Resonance Energy Transfer, FRET). So too see if two proteins interact, one can tag each with different fluorescent markers that are excited by different light wavelengths. When the first fluorescent marker ( say a green marker) absorbs light energy, it can transfer energy by FRET to the second marker ( eg.a red marker), but ONLY if they are very close together ( within 10 nm). So if the proteins are far apart, when the green tag is illuminated, it can only re- emit green light. If the proteins ( and hence the markers) are close together however, the green marker can pass on energy by FRET to the red marker, and red light will be seen. I am constantly amazed at the methods designed by biologists to answer questions!
After quite a marathon of talks, with a short break for coffee, the evening gave way to a discussion on the future of publishing in plant science, chaired by a panel of journal editors. Professor Mike Blatt, editor of Plant Physiology, made the point that many people often assume that scientific journal editors are 'failed scientists' who couldn't handle the pressures of a research- based career. On the contrary, most journal editors are very much 'active' scientists, who manage to fit in editing and reviewing with their own research agendas. There was much debate on how the move towards 'open access' publishing can be financially sustained. Although there is great pressure for publicly-funded research to be made freely available, most journals receive most of their revenue from subscriptions. Without this income, how can we ensure that these remain high quality and rigorously reviewed publishing platforms? Eventually, the talk was pulled to a close and we tripped onto the lane for the drinks reception. The formal meal was very enjoyable, the highlight for me being 'Japanese breaded baby carrots' although the menu didn't specify what exactly made the dish 'Japanese' - were they Panko breadcrumbs? Did it refer to the way the carrots had been exquisitely julienned? Were the carrots themselves from Japan?
The next morning kicked off with an open platform to discuss strategies to inspire plant science teaching in schools. I can remember from my GCSEs that my teachers were almost apologetic when we reached plant topics, with an air of 'I'm sorry but we HAVE to do this because it's on the exam...'. To combat this, Science And Plants in Schools (SAPS) have produced a stunning suite of freely available teaching resources ( including lesson plans, animations, work sheets) - the problem is now getting the message out there so that teachers actually use them!
After more talks from the PhD students, the meeting closed with a presentation by Professor Cathie Martin of The John Innes Centre, to describe the potential of nutrient enhanced food to combat chronic diseases. Her work has mainly focused on the purple tomato; a GM crop with a introduced pigment gene from Antirrhinum ( snapdragon). This increases the production of anthocyanins, said to protect against a range of chronic diseases. To test this, the tomatoes were fed to mice genetically engineered to be deficient in the tumour suppressor p53. This dramatically reduces the lifespans of the mice to an average of 140 days. Supplementing the mice's diet with red tomatoes had little effect, however introducing purple tomatoes caused them to live 30% longer, up to 180 days. The tomatoes were also tested in atherosclerosis - prone mice, where it was found that, as the proportion of purple tomatoes in their diet increased, the area of fatty plaque on the arteries reduced. European regulations mean that these 'wonder fruits ' are currently only available as a juice in the United States. Professor Martin's group are currently seeking to develop tomatoes enriched in resveratol ( a compound found in red grapes claimed to promote longevity and weight loss) and phytoestrogens ( which could benefit women approaching the menopause). Having an abundance of bio enriched foods sounds wonderful, however Professor Martin pointed out one can't rely on these foods alone for good health. The plaque reducing properties of purple tomatoes for instance, would be severely negated by pairing them with melted cheese on a pizza! It was interesting though, that the plums were the first fruit to run out over the leaving lunch....
How the years roll by... At least it means I can count on the next meeting being here before I know it! So long for now!



